LUNARIS™ Mouse 12-Plex Th17 Kit

The 12 biomarkers of this panel are involved in regulating the differentiation and effector functions of murine Th17 cells and have human analogs, making this kit a powerful tool for connecting insights from cell and animal models to human biology. Measurement of active transforming growth factor β1 (TGF-β1) plus interleukins IL-6 and IL-23, is complemented with sensitive quantification of IL-1β, IL-4, IL-17A, IL-17E, IL-17F, IL-21, CCL20, and interferon-γ (IFN-γ).

LUNARIS™ Kits include all components necessary to perform the assay and readout on the LUNARIS™ Reader: LUNARIS™ BioChips, diluents, buffers, reagents, standards, LUNARIS™ Decoding File, LUNARIS™ Analysis Suite, and instructions for use.

mouse Th17

Specifications

CCL20 IFN-Ɣ IL-10 IL-17A IL-17E IL-17F IL-1β IL-21 IL-23 IL-4 IL-6 TGF-β1

Autoimmune disorders, immune-mediated diseases, 
allergies, cancer, microbial defense and transplantation,
rheumatoid arthritis, multiple sclerosis

Mouse
Cell culture supernatant
Serum

LUNARIS™ Products are research use only, not for use in diagnostic procedures.

Excellent Data Quality

performance Th17

LUNARIS Mouse 12-Plex Th17 Kit
Exceptional assay sensitivity and robustness result in a broad dynamic range. Graph bars span from the limit of detection (LoD) to the upper limit of quantification (ULOQ) for each analyte. The white line indicates the lower limit of quantification (LLOQ). Values represent the median of 20 experiments.

recoveries Th17

LUNARIS Mouse 12-Plex Th17 Kit
High recoveries of all panel analytes. The graph on the left summarizes the analysis of ten experiments quantifying markers in quality control samples at three different concentrations spiked into cell culture supernatants. Median recovery (orange circles) and standard deviation are given for each tested analyte. The gray area denotes the targeted recovery range 70–130%.

precision Th17

LUNARISMouse 12-Plex Th17 Kit
Superior between-run precision delivers reliable analyte quantification. Quality control samples at three different  concentrations were spiked into cell culture supernatants and markers were quantified in ten experiments. Orange circles represent the median between-run precision of each tested analyte. The gray area denotes the desired precision (<20%).

Kit Format

LUNARIS™ Kits are ready-to-use solutions and supply all components to start your multiplex protein experiment immediately.

Each kit is available in two formats to meet your specific throughput needs.

The 96-well density microtiter plate format accommodates up to three LUNARIS™ BioChips, each with 32-wells. Test eight samples on a single BioChip or up to 40 samples on the complete 96-well assembly.

The 384-well density microtiter plate format accommodates up to four 96-well LUNARIS™ BioChips. Test up to 40 samples on a single BioChip or up to 184 samples in duplicate when using all four BioChips.

96 well
384 well

Ordering

LUNARIS™ Mouse 12-Plex Th17 Kit

MTP Plate Format No. of BioChips Cat. No. No. of Samples*
96 1 x 32 LMTH-10120S 8
96 3 x 32 LMTH-10120F 40
384 1 x 96 LMTH-20120S

40

384 4 x 96 LMTH-20120F 184

*Measured in duplicate

Tailored Testing and Readout Services

services

Outsource the labwork and focus on results. Our service experts can take over some or all the testing and readout steps. Perform the assay in your lab and send us your BioChips for readout and analysis, or engage our full service, from sample to result.

Interested in a tailored panel with your own or commercially available antibodies? Talk to our experts about our customer panel development service.

Read more / LUNARIS™ Services

Related Kits

LUNARIS™ Technology

The power of our proprietary LUNARIS™ technology lies in its elegant simplicity, which we leveraged to engineer an integrated and scalable multiplex protein analysis system, with a dedicated reader and intuitive image-based readout and data analysis.

The result is a platform that delivers transparent assay outcomes, superior data quality, workflow flexibility, and conservation of precious samples. You quantify as many as 12 biomarkers in just 3 µl of sample. You adapt the system to your throughput needs. You decide when to perform readout and how to evaluate each data point.

LUNARIS™ embodies our vision of success in translational proteomics. Validated for various biofluids, tissue samples, and cell culture, LUNARIS™ opens new opportunities in multiplex biomarker quantification to drive advances from model to man, from lab to clinic, from data to insight.

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