LUNARIS™ Human 11-Plex Ophthalmology Kit

A panel of mediators in retinal angiogenic diseases and key inflammatory markers relevant to ophthalmic conditions. Quantify three chemokines (CXCL), angiopoietin-2 (Ang-2), monocyte chemoattractant protein-1 (MCP-1/CCL2), C-reactive protein (CRP), platelet-derived growth factor-BB (PDGF-BB), placental growth factor (PlGF), vascular endothelial growth factor-A (VEGF-A), plus interleukins IL-6 and IL-8 in human ocular fluids, plasma, or tears.

LUNARIS™ Kits include all components necessary to perform the assay and readout on the LUNARIS™ Reader: LUNARIS™ BioChips, diluents, buffers, reagents, standards, LUNARIS™ Decoding File, LUNARIS™ Analysis Suite, and instructions for use.

LUNARIS 11-Plex Ophthalmology Kit

Specifications

Ang-2 CCL2 CRP CXCL10 CXCL12 CXCL13 IL-6 IL-8 PDGF-BB PlGF VEGF-A

Age-related macular degeneration (AMD),
proliferative diabetic retinopathy (PDR),
diabetic macular edema (DME)

Human
Aqueous humor
Plasma
Tears
Vitreous humor

LUNARIS™ Products are research use only, not for use in diagnostic procedures.

Excellent Data Quality

LUNARIS Human 11-Plex Ophthalmology Kit - Assay Performance

LUNARIS™ Human 11-Plex Ophthalmology Kit
Exceptional assay sensitivity and robustness result in a broad dynamic range. Graph bars span from the limit of detection (LoD) to the upper limit of quantification (ULOQ) for each analyte. The white line indicates the lower limit of quantification (LLOQ). Values represent the median of 20 experiments.

LUNARIS Human 11-Plex Ophthalmology Kit - Precision

LUNARIS™ Human 11-Plex Ophthalmology Kit
Superior between-run precision delivers reliable analyte quantification. Quality control samples at three different concentrations were spiked into cell culture supernatants and markers were quantified in ten experiments. Orange circles represent the median between-run precision of each tested analyte. The gray area denotes the desired precision (<20%).

LUNARIS Human 11-Plex Ophthalmology Kit - Dilution Linearity

LUNARIS Human 11-Plex Ophthalmology Kit
Accurate assay performance over a range of dilutions. Vitreous humor samples spiked with VEGF-A were serially diluted prior to quantification. The above graph plots measured VEGF-A concentration against dilution factor to demonstrate near perfect linearity.

Kit Format

LUNARIS™ Kits are ready-to-use solutions and supply all components to start your multiplex protein experiment immediately.

Each kit is available in two formats to meet your specific throughput needs.

The 96-well density microtiter plate format accommodates up to three LUNARIS™ BioChips, each with 32-wells. Test eight samples on a single BioChip or up to 40 samples on the complete 96-well assembly.

The 384-well density microtiter plate format accommodates up to four 96-well LUNARIS™ BioChips. Test up to 40 samples on a single BioChip or up to 184 samples in duplicate when using all four BioChips.

96 well
384 well

Ordering

LUNARIS™ Human 11-Plex Ophthalmology Kit

MTP Plate Format No. of BioChips Cat. No. No. of Samples*
96 1x32 LHOP-10110S 8
96 3x32 LHOP-10110F 40
384 1x96 LHOP-20110S 40
384 4x96 LHOP-20110F 194

*Measured in duplicate

Tailored Testing and Readout Services

services

Outsource the labwork and focus on results. Our service experts can take over some or all the testing and readout steps. Perform the assay in your lab and send us your BioChips for readout and analysis, or engage our full service, from sample to result.

Interested in a tailored panel with your own or commercially available antibodies? Talk to our experts about our customer panel development service.

Read more / LUNARIS™ Services

Related Kits

LUNARIS™ Technology

The power of our proprietary LUNARIS™ technology lies in its elegant simplicity, which we leveraged to engineer an integrated and scalable multiplex protein analysis system, with a dedicated reader and intuitive image-based readout and data analysis.

The result is a platform that delivers transparent assay outcomes, superior data quality, workflow flexibility, and conservation of precious samples. You quantify as many as 12 biomarkers in just 3 µl of sample. You adapt the system to your throughput needs. You decide when to perform readout and how to evaluate each data point.

LUNARIS™ embodies our vision of success in translational proteomics. Validated for various biofluids, tissue samples, and cell culture, LUNARIS™ opens new opportunities in multiplex biomarker quantification to drive advances from model to man, from lab to clinic, from data to insight.

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