Characterization of the inflammatory cytokine and growth factor profile in a rabbit model of proliferative vitreoretinopathy
Wong C.W., Cheung N., Ho B., Barathi V., Storm G., Wong T.T.; Scientific Reports (9): 15419-26 (2019)
Proliferative vitreoretinopathy (PVR) is a blinding condition that can occur after ocular trauma, retinal detachment, or surgery for retinal detachment repair. Though known to involve inflammation as cells migrate into and proliferate in the vitreous humor of eyes, the role that cytokine and growth factor secretion plays in the pathobiology of PVR remains unclear.
Therefore, scientists at the Singapore Eye Research Institute (SERI) established rabbits as an animal model for PVR and published a study monitoring changes in cytokine and growth factor levels in vitreous humor associated with PVR over time. Their work shows a spike in inflammation cytokines two weeks after surgical induction of PVR, followed by rising fibroproliferative growth factors likely to support the survival and epithelial mesenchymal transition of retinal pigment epithelial cells.
Repeated sample drawings from the same animals meant that each sample taken had to be minute. With a meaningful biomarker panel and extremely low sample input, the LUNARIS™ Human 11-Plex Ophthalmology Kit enabled the authors to profile 11 analytes from each sample taken at every time point. This work by our research partner SERI represents a critical step in advancing model-to-man translational research in ophthalmology.